J S Hernández’s research while affiliated with Mayo Clinic – Rochester – Minnesota – USA
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Sulfation of estrone and 17β-estradiol in human liver: Catalysis by thermostable phenol sulfotransferase and by dehydroepiandrosterone sulfotransferaseArticle
Sulfation is a major pathway in humans for the biotransformation of estrogens. However, the nature of the enzymes that catalyze the sulfation of estrone (E1) and 17 beta-estradiol (E2) in human liver is unclear. Human liver contains at least three well-characterized cytoplasmic sulfotransferases, the thermostable (TS) and thermolabile (TL) forms of…CiteRequest full-textHuman liver arylamine N-sulfotransferase activity. Thermostable phenol sulfotransferase catalyzes the N-sulfation of 2-naphthylamineArticle
Our experiments were performed to determine whether human liver, like that of other mammals, could catalyze the N-sulfation of an arylamine, 2-naphthylamine (2-NA) and, if so, whether this reaction might be catalyzed by one or both of the two known forms of human phenol sulfotransferase (PST). One form of PST is thermostable (TS) and catalyzes the…CiteRequest full-textThiopurine methyltransferase regulation in rat kidney: Immunoprecipitation studiesArticle
1. Thiopurine methyltransferase (TPMT) catalyses the S-methylation of thiopurine drugs. TPMT activity in the kidneys of male Sprague-Dawley (S-D) rats is approximately twice that present in the kidneys of female S-D rats, and this difference is testosterone-dependent. Renal TPMT activities in these animals also increase dramatically during growth a…CiteRequest full-textHuman liver estrone (E1), Estradiol (E2) and dehydroepiandrosterone (DHEA) sulfotransferases (STs): Comparison with thermostable (TS) and thermolabile (TL) phenol sulfotransferase (PST) activitiesArticle
Sulfation plays an important role in the metabolism of E1, E2 and DHEA in humans. The relationship between the enzymes that catalyze the sulfation of E1, E2 and DHEA and TS and TL PST is unclear. The authors compared thermal stability, sensitivity to inhibition by 2,6-dichloro-4-nitrophenol (DCNP) and individual variation in the regulation of these…CiteRequest full-textMouse thiopurine methyltransferase pharmacogenetics: Correlation of immunoreactive protein and enzymatic activityArticle
Thiopurine methyltransferase (TPMT) catalyzes the S-methylation of aromatic and heterocyclic sulfhydryl compounds including thiopurine drugs such as 6-mercaptopurine. Genetic polymorphisms control levels of TPMT enzymatic activity in both humans and inbred mice. AKR/J (AK) and C57BL/6J (B6) mice have genetically low, whereas DBA/2J (D2) mice have g…CiteRequest full-text
… However, differential cell counts performed on bone marrow smears from AZA-treated mice (Table 5) did not show a reduced number of erythroid cells. TPMT genetic polymorphisms which play a role in the development of bone marrow injury following AZA treatment in man also occur in some strains of mouse (Hernandez et al. 1990). It is possible that if the present studies had been conducted with a strain of mouse that is known to have a genetically low TMPT activity (for example, the C57BL ⁄ 6J or AKR ⁄ J strains) a more acute bone marrow injury may have been observed involving all haemopoietic cell lineages. …Reference: The haemotoxicity of azathioprine in repeat dose studies in the female CD‐1 mouseMouse thiopurine methyltransferase pharmacogenetics: Correlation of immunoreactive protein and enzymatic activityCiting article
View… Most, if not all, cellular effects of estrogens are mediated by the nuclear receptors estrogen receptor alpha (ERα) and -beta (ERß) with a high ligand-receptor binding affinity (Kd˜1nM) . Although estrogens have been reported to be the substrates of multiple SULTs including SULT1A1 and SULT2A1 , SULT1E1 exhibits the highest affinity for these hormones, especially the 3-hydroxyl position of E 2 [5,29], to which it binds with a Michaelis-Menten constant (Km) of 0.27 nM and with a turnover number (kcat) of 10s −1 x10 3 /nM respectively [31,32]. This sulfoconjugation of estrogens can be reversed by the deconjugation reaction catalyzed by the steroid sulfatase (STS) . …Reference: Hepatic Estrogen Sulfotransferase Distantly Sensitizes Mice to Hemorrhagic Shock-Induced Acute Lung InjurySulfation of estrone and 17β-estradiol in human liver: Catalysis by thermostable phenol sulfotransferase and by dehydroepiandrosterone sulfotransferaseCiting article
View… In human liver, the N-sulfonation of various alicyclic amines is mediated primarily by SULT2A1 (Shiraga et al. 1999a). SULT1A1 appears to be the major enzyme catalyzing the sulfonation of 2-naphthylamine (Hernandez et al. 1991). …Reference: Pharmacogenetics of soluble sulfotransferases (SULTs). Naunyn Schmiedebergs Arch PharmacolHuman liver arylamine N-sulfotransferase activity. Thermostable phenol sulfotransferase catalyzes the N-sulfation of 2-naphthylamineCiting article
Top co-authors (7)
- Mayo Foundation for Medical Education and Research
Thomas C Wood
- Mayo Foundation for Medical Education and Research
Jon A. Van Loon
D M Otterness
R. W. G. Watson
S P Powers
Drug Metabolism and Disposition: the Biological Fate of Chemicals (2)Xenobiotica (1)Journal of Pharmacology and Experimental Therapeutics (1)The FASEB Journal (1)
Mayo Clinic – RochesterDepartment
- Department of Molecular Pharmacology and Experimental Therapeutics
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